Figure 3 | Cell Death & Disease

Figure 3

From: Increased tumor-infiltrating CD45RA−CCR7− regulatory T-cell subset with immunosuppressive properties foster gastric cancer progress

Figure 3

Tumor-derived TNF-α induces CD45RA−CCR7− Treg subset via STAT3 phosphorylation. (a,b) Dot plots and statistics analysis of CD45RA−CCR7+ and CD45RA−CCR7− Treg subset after Tregs exposed to TTCS for 24 h with or without pre-treated with different concentrations of STAT3 phosphorylation inhibitor FLLL32 for 1 h. (c) STAT3 and p-STAT3 proteins in Tregs exposed to autologous TTCS, or TTCS with anti-TNF-α antibody or TTCS pre-treated with FLLL32 (an STAT3 inhibitor) or DMSO were analyzed by western blot. (d) Dot plots and HLA-DR expression of CD45RA−CCR7+ and CD45RA−CCR7− Treg subset after Tregs exposed to TTCS for 24 h with or without pre-treated with STAT3 phosphorylation inhibitor FLLL32 for 1 h. *, P<0.05; **, P<0.01, and n.s., P>0.05 for groups connected by horizontal lines

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