Figure 4 | Cell Death & Disease

Figure 4

From: Increased tumor-infiltrating CD45RA−CCR7− regulatory T-cell subset with immunosuppressive properties foster gastric cancer progress

Figure 4

CD45RA−CCR7− Treg subset suppress CD8+ T-cell functions via IL-10 secretion and cell–cell contact mechanisms. (a) Representative surface staining and statistics analysis of the expression of HLA-DR on CD45RA−CCR7− (blue), CD45RA−CCR7+ (red) or CD45RA+CCR7− (green) Treg subsets. (b) IL-10 concentration between autologous tumor and non-tumor tissues (n=19) was analyzed. (c and d) CD8+ T cells and Treg subsets were co-cultured and assessed by transwell assay as described in Materials and Methods. Representative data (c) and statistical analysis (d) of CD8+ T-cell proliferation and IFN-γ, GrB production were shown (n=3). (e) The correlations between IL-10 and CD45RA−CCR7− Treg subset in GC were analyzed. Results are expressed as percentage of CD45RA−CCR7− Treg subset in total Tregs or the number of CD45RA−CCR7− Treg subset per million total cells and IL-10 concentration in gastric tumor tissues. The horizontal bars and each dot in panel a represent mean values and one patient. *, P<0.05; **, P<0.01, and n.s., P>0.05 for groups connected by horizontal lines

Back to article page