Figure 5

Blockade of immunosuppressive CD45RA⁻CCR7⁻ Tregs inhibits tumor growth and GC progression in vivo. (a) Mice were injected with human SGC7901 cells, as described in Materials and methods. The control animals (●) received no further injections. The experimental treatments entailed injections with CD8⁺ T cells alone (○) or in combination with TTCS-conditioned CD45RA⁻CCR7⁻ Tregs with (▴) or without (▾) pre-coculturing, or TTCS-conditioned CD45RA⁻CCR7⁻ Tregs pre-treated with an anti-IL-10 antibody (▾) or a control IgG (▴). The illustrated data represent tumor volumes (five mice in each group). The day of T-cell injection was counted as day 0. The tumors were excised and photographed 19 day after injecting T cells. (b–e) IFN-γ-producing T-cell response (b) in spleens, proliferating cell nuclear antigen (PCNA) (brown) expression or CD8⁺ T-cell infiltration (red) (c), and production (d) or expression (e) of IFN-γ and IL-10 in tumors of mice on day 19 after T-cell injection were compared. Scale bars: 100 μm. *P<0.05; **P<0.01, and n.s., P>0.05 for groups connected by horizontal lines