Figure 6 | Cell Death & Disease

Figure 6

From: Amlexanox, a selective inhibitor of IKBKE, generates anti-tumoral effects by disrupting the Hippo pathway in human glioblastoma cell lines

Figure 6

IKBKE interacts with and degrades LATS2. (a) Expression of LATS1/2 in IKBKE knockdown and control cells was examined at transcript levels. (b) The endogenous interaction between IKBKE and LATS1/2 in U87 cells was analyzed by immunoprecipitation. (c) LATS2 expression in IKBKE-knockdown U87 cells and control cells was measured after treatment with or without 20 μM MG132 for 12 h. (d) Decreased LATS2 ubiquitylation level by IKBKE knockdown in U87 cells. (e) Measurement of LATS2 in cell lysates harvested at 0, 2, 4, 8 and 12 h after the addition of CHX (100 μM) to arrest protein synthesis. (f) Schematic diagram of the mechanism of amlexanox-mediated antitumor activity by downregulation of IKBKE in GBM. IKBKE directly binds to and negatively regulates LATS1/2, which promotes YAP1 cytoplasmic retention and subsequent degradation

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