Figure 3
From: TNFα deficiency results in increased IL-1β in an early onset of spontaneous murine colitis
TNFα KO Winnie mice show an increased Th1 signature at the molecular level. mRNA was extracted from the colon of all the 5-week-old mice genotypes. The expression level of Il1β, Ifnγ, Il12, Il10 and Il4 was measured by qPCR and normalized to the housekeeping gene Gapdh (a, b, c, d, e, respectively). Bars represent mean relative expression±S.E.M. (n=4) for each genotype. Statistical evaluation between C57/Bl6 mice and each murine line was performed using unpaired two-tailed Student’s t-test. ***P<0.001, **P<0.01, *P<0.05
