Figure 6

IL-1β upregulation in TNFα KO BMDCs. BMDCs were cultured from WT (black bars) and TNFα KO (white bars) mice, treated with TNFα and anti-TNFα antibody at day 7 and concomitantly exposed to 1 μg/ml of LPS. Twenty-four hours later, the secretion of IL-1β was determined by ELISA (a). Bars represent mean cytokine concentration±S.E.M. (n=3). For each treatment statistical evaluation between WT and TNFα KO DCs was performed using two-way ANOVA and Bonferroni as post-test. For TNFα KO DCs treated with LPS, paired two-tailed Student’s t-test was conducted to compare BMDCs treated or untreated with TNFα. The expression level of Il1β was measured in WT and TNFα KO DCs by qPCR and normalized to the housekeeping gene Gapdh. Bars represent mean relative expression±S.E.M. (n=4) (b). Statistical evaluation between WT and TNFα KO DCs was performed using unpaired two-tailed Student’s t-test. *P<0.05, **P<0.01, ***P<0.001