Figure 4
Intracellular FGF1WT protects SH-SY5Y cells from p53-dependent apoptosis. Stably transfected SH-SY5Y cells with either FGF1WT or empty (mock) expression vectors were treated or not with etoposide for 16 h. (a) Polyclonal transfected SH-SY5Y apoptotic cells were characterized by flow cytometry after DiOC6(3) and PI staining (apoptotic cells are the DIOC−, PI− and size− cells). The graph represents the mean±S.E.M. of three independent experiments. Student’s t-tests were performed relative to the mock control, except where indicated (n=3; n.s.: P>0.05; ***: P⩽0.001). (b) FGF1 expression and p53 activation (phosphorylated Ser15) were assessed by western blot in either polyclonal transfected cells (left panel) or isolated transfected cell lines (right panel). Actin detection was used as control. (c) FGF1 expression, caspase-9 and -3 cleavages were assessed in polyclonal transfected cells by western blot. Actin detection was used as control
