Figure 1

Cardamonin inhibits proliferation and division of NPC cells. (a) CNE-1, CNE-2, HONE-1 and SUNE-1 cells were treated with different concentrations of cardamonin or 0.1% DMSO for 24 h. Cellular viability was measured with a CCK-8 assay, **P<0.01, n=3. (b) NPC cells were treated with 10 μM cardamonin or 0.1% DMSO, absorbance was measured after 24, 48 and 72 h of treatment. (c) CNE-2 cells were cultured for 7 days after treatment with varying concentration of cardamonin, and stained with 0.1% crystal violet. Colonies containing >50 cells were counted, **P<0.01, n=3. (d) CFSE labeled cells were measured using flow cytometry after treatment with cardamonin. **P<0.01, n=3