Figure 2 | Cell Death & Disease

Figure 2

From: Cardamonin induces ROS-mediated G2/M phase arrest and apoptosis through inhibition of NF-κB pathway in nasopharyngeal carcinoma

Figure 2

Cardamonin induces apoptosis and G2/M phase arrest in CNE-2 cells. (a) CNE-2 cells were cultured with indicated concentrations of cardamonin for 24 h, apoptosis was measured using Annexin V-FITC and PI staining method. **P<0.01, n=3. (b) CNE-2 cells were treated with 20 μM of cardamonin for different time, apoptosis was measured using Annexin V-FITC and PI staining method. **P<0.01, n=3. (c) Bid, Bax, Cleavage of caspase 8, caspase 8 and PARP were evaluated by western blot. (d) Cell cycle analysis after treatment with varying concentrations of cardamonin for 24 h. **P<0.01. (e) The protein expression of p53, p21 and Cyclin D1 was evaluated by western blotting after treatment with cardamonin

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