Figure 3
SDF1 protects and induces migration of DOXO treated CmPC. (a) Cell death analysis of CmPC upon DOXO treatment for increasing periods of time starting from 6 h up to 48 h as indicated. Cell death increased significantly 48 h after DOXO treatment (n=4, ***P<0.001). (b) Cell death analysis of CmPC exposed or not to 1 μM DOXO. DOXO was added to CmPC for 24 h and then removed. SDF1, AMD3100 and α-CXCR4 were added after 24 h at the following dosages: SDF-1 at 100 ng/ml, AMD3100 at 3.2 μg/ml and α-CXCR4 at 10 μg/ml. Cell death was determined at 48 h. Mean values±SEM of three independent experiments run in triplicate (*P<0.05; ***P<0.0001). (c) Chemotatic responses of CmPC exposed or not to 1 μM DOXO for 24 h in response to 100 ng/ml SDF1 or 10% FBS. Migration efficiency of CmPC was estimated using a Transwell assay. After 16 h of incubation transmigrated cells were quantified using crystal violet. The results are expressed as fold change of the untreated control cells exposed or not to DOXO. DOXO—cells treated with 1 μM DOXO for 24 h; AMD3100—serum starved CmPC treated AMD3100 25 μg/ml during migration; DOXO+AMD3100—cells treated with 1 μM DOXO for 24 h and AMD3100 25 μg/ml during migration (n=6, *P<0.05; **P<0.005; ***P<0.0001)
