Figure 4

DOXO treatment upregulates miR-200c and downregulates ZEB1. (a) miR-200c expression upon 24 h DOXO treatment. miR-200c was significantly induced by DOXO treatment (n=4, *P<0.05). Results are presented as mean±S.E.M. (b) ZEB1 mRNA expression was quantified by qPCR; ZEB1 mRNA decreased upon DOXO treatment at 24 h (n=4, **P<0.01). Results are presented as mean±S.E.M. (c) CmPC were treated with 1 μM DOXO for 24 h. ZEB1 and p53 proteins were evaluated by Western blot analysis. A representative western blot showing DOXO treatment induced p53 protein expression and ZEB1 downregulation. β-actin was used as a loading control. Actin indicates β-actin (d) Expression levels of ZEB1 and p53 protein were evaluated by densitometric analysis and normalized by β-actin protein levels (n=3, *P<0.05)