Figure 5

ZEB1 knockdown elicits CXCR4 increase. CmPC were infected either with the lentivirus carrying ZEB1-specific shRNA or with the control virus. After 24 h, cells were selected with puromycin. (a) Representative western blot demonstrating a 60% knockdown of ZEB1 expression in CmPC infected with a lentivirus encoding a ZEB1-specific shRNA sequence. (b) Expression levels of ZEB1 protein were evaluated by densitometric analysis and normalized by β-actin protein levels (n=3, *P<0.02). (c) CmPC transduced with a ZEB1-specific shRNA showed an upregulation of CXCR4 mRNA compared with control. (d) CmPC transduced with a ZEB1-specific shRNA displayed CXCR4 protein expression on the cell surface of CmPC, and increased both in absence of DOXO (upper and bottom left panels) and in presence of DOXO (upper and bottom right panels)