Figure 6
CXCR4 is a ZEB1 target gene. (a) Schematic figure showing the E-box binding sites of ZEB1 in the promoter and in the intronic region of the human CXCR4 gene. Five E-box sites are present at position – 2029 bp, −1644 bp, −983 bp, −554 bp, −262 bp upstream the first exon and four in the intronic region, at position +881 bp, +916 bp, +1238 bp, +1454 bp. (b) ZEB1 consensus sites revealed that the E-box sites at +881 bp and +916 bp are highly conserved across species of humans, mice, rats, rabbits, bos taurus and pan troglodytes. (c) ChIP assay of CmPC in the absence or presence of 1 μM DOXO treatment for 24 h was performed with a ZEB1 antibody, followed by quantitative real-time PCR (ChIP-qPCR), using specific primers encompassing ZEB1 consensus sequences. (d) ChIP-qPCR CmPC in the absence or presence of 1 μM DOXO treatment for 24 h was performed with a Pol II-specific antibody
