Figure 8
SDF1 partially rescues DOXO-dependent cardiac dysfunction via a miR-200c/ZEB1/p53 pathway modulation. Mice were treated with DOXO, DOXO+SDF1 or saline for 21 days. (a) Then mRNA was extracted from LV and analyzed for miR-200c, p53, ZEB1 and p21 mRNA expression levels. p53 mRNA was not modulated either by DOXO or DOXO+SDF1. miR-200c, and p21 mRNA were induced by DOXO and were all significantly decreased by SDF1 treatment. ZEB1 mRNA was downregulated by DOXO and returned to control levels by SDF1 treatment (Saline, n=5; DOXO, n=5; DOXO+SDF1, n=5; *P<0.05; **P<0.01; ***P<0.001). (b) Representative western blot with anti-p53 and ZEB1 antibodies showed that p53 upregulation by DOXO was decreased by SDF1 treatment and ZEB1 demise was reverted by SDF1 (Saline, n=5; DOXO, n=5; DOXO+SDF1, n=5). (c) Expression levels of p53 and ZEB1 protein were evaluated by densitometric analysis and normalized by β-actin protein levels (n=5; *P<0.05; ***P<0.001)
