Figure 3

Hypoxia decreases AR by upregulating HIF-2α. (a,b) Western blot assays showed that cobalt chloride and Oxyrase treatments could suppress AR expression on HepG2 and SKhep1 as the treatment being prolonged. (c) Western blot assays showed exposure to hypoxia (1% oxygen) could significantly suppress AR expression in both HepG2 and SKhep1 cells. (d) Western blot assays showed knocking down of HIF-2α could partially rescued the AR decreased by hypoxia