Figure 2

Cezanne expression level correlated with the expression of epithelial–mesenchymal transition (EMT) markers. (a) Serial sections of human HCC tissues were subjected to IHC staining with antibodies against Cezanne, E-cadherin and vimentin. In case 1, high expression of Cezanne in HCC tissues was accompanied by elevated E-cadherin and the absence of vimentin. In case 2, low expression of Cezanne was accompanied by the absence of E-cadherin and elevated vimentin. The scale bar represents 50 μm. (b) Cezanne expression was positively correlated with E-cadherin expression and negatively associated with vimentin expression. (c and d) Transfecting siRNAs against Cezanne decreased Cezanne expression, downregulated E-cadherin and upregulated vimentin. Overexpression of Cezanne upregulated E-cadherin and downregulated vimentin. (e) Representative images of IHC staining of mice xenografts of SK-Hep1 sh-Cezanne and control cells. The scale bar represents 50 μm