Figure 3

Immunofluorescence staining showed the protein levels of RUNX2, SIRT7, and β-catenin. (a) The level of RUNX2 protein (red) at day 3 of osteogenic differentiation. (b) The level of SIRT7 protein (red) at day 3 of osteogenic differentiation. (c) The level of β-catenin protein (red) at day 3 of osteogenic differentiation. The nuclei were counterstained with DAPI (4′,6-diamidino-2-phenylindole; blue). All the data were confirmed by three repeated tests. Scale bar=100 μm