Figure 1

Rg5 prevented acidification in cardiomyocytes. Neonatal rat ventricular myocytes (NRVMs) were stimulated with palmitate (PA, 100 μM) for 2 h. (a and b) Lactate contents (n=5) and NADH/NAD+ ratio (n=5). (c) Extracellular acidification ratio (ECAR) in NRVMs was measured using an XFe96 Extracellular Flux Analyzer (n=4). (d) CPT-1 protein expression in NRVMs was determined with western blot (n=3). (e) Oxygen consumption ratio (OCR) was measured using an XFe96 Extracellular Flux Analyzer (n=4). (f) Phosphorylation of PDH was detected with western blot (n=3). Data are expressed as mean±S.D. P<0.05 versus PA treatment; #P<0.05 versus indicated treatment