Figure 4 | Cell Death & Disease

Figure 4

From: Ginsenoside Rg5 increases cardiomyocyte resistance to ischemic injury through regulation of mitochondrial hexokinase-II and dynamin-related protein 1

Figure 4

Rg5 inhibited Drp1 activation in cardiomyocytes. Neonatal rat ventricular myocytes (NRVMs) were stimulated with palmitate (PA, 100 μM) for 2 h. (a) Confocal image of Drp1 in mitochondria (Green: Drp1; Red: MitoTracker Red cMXRos; Scale bars: 10 μm). (b) Mitochondrial fission was viewed by Mito Tracker Red CMXRos with confocal microscopy (Upper, scale bar, 10 μm; Lower, magnified images, bar, 5 μm). (c) The phosphorylation of Drp1 (S637) in NRVMs was determined by western blot; *P<0.05 versus the control. (d) Drp1 phosphorylation (S637) in NRVMs treated with PA for 2 h. *P<0.05 versus PA treatment; #P<0.05 versus indicated treatment. (e) Drp1 phosphorylation (S637) in H9c2 cells transfected with Akt2 or control scrambled siRNA was determined by western blot. Data are expressed as mean±S.D. from three independent experiments. *P<0.05 versus control siRNA+PA treatment; #P<0.05 versus indicated treatment

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