Figure 5 | Cell Death & Disease

Figure 5

From: Ginsenoside Rg5 increases cardiomyocyte resistance to ischemic injury through regulation of mitochondrial hexokinase-II and dynamin-related protein 1

Figure 5

Regulation of Drp1 and HK-II in mitochondria. Neonatal rat ventricular myocytes (NRVMs) were stimulated with palmitate (PA,100 μM) for 2 h. (a and b) Immunoprecipitation and western blot examination of PAS and Akt in precipitated Drp1 protein. *P<0.05 versus PA treatment; #P<0.05 versus indicated treatment. (c) Confocal scanning image of mitochondrial fission (Mito Tracker Red CMXRos). Upper, scale bar, 10 μm; Lower, magnified images, bar, 5 μm. (d) Confocal scanning image of mitochondrial HK-II expression in H9c2 cells transfected with Drp1 or control scrambled siRNA (Green: HK-II; Red: MitoTracker Red CMXRos; Upper, scale bar, 10 μm; Lower, magnified images, bar, 5 μm) (e and f) Confocal scanning image of mitochondrial Drp1 expression in NRVMs pretreated with 2-deoxy-D-glucose (2-DG, 5 mM) for 24 h or insulin (0.1 μM) 30 min (Green: Drp1; Red: MitoTracker Red cMXRos; Scale bars: 10 μm). Data are expressed as mean±S.D. (n=3). The results are confirmed by three independent experiments

Back to article page