Figure 7
Rg5 increased cardiomyocyte resistance to I/R injury. Neonatal rat ventricular myocytes (NRVMs) were stimulated with palmitate (PA, 100 μM) for 2 h before 4 h hypoxia (1% O2) and 1 h reoxygenation (H/R). (a) Intracellular ROS production in cardiomyocytes was viewed by dihydroethidium staining labeling with confocal microscopy. Scale bar, 100 μm. The results are confirmed by three independent experiments. Intracellular calcium concentration (b) and ATP content (c) were detected by commercial kits (n=4). (d) The level of cytochrome C in mitochondrial and cytosol fraction were measured by ELISA (n=5). (e) The apoptosis assay was performed with AnnexinV/PI staining by flow cytometry analysis (n=3). Data are expressed as mean±S.D. *P<0.05 versus PA with H/R treatment; #P<0.05 versus indicated treatment. (f) The proposed mechanism by which Akt activation prevented Drp1 recruitment and mitochondrial HK-II dissociation in cardiomyocyte
