Figure 2 | Cell Death & Disease

Figure 2

From: p73 is required for appropriate BMP-induced mesenchymal-to-epithelial transition during somatic cell reprogramming

Figure 2

Lack of p73 impairs MET resulting in altered maturation and stabilization phases. (a) Overview of the experimental design of MEFs reprogramming by doxycycline inducible OSKM transfection and sample collection during the initiation, maturation and stabilization phases of the process. (b and c) Analysis of the expression kinetics profile of markers during the reprogramming process of WT (b and c) and p73KO (c) MEFs. RNA samples were collected at the indicated days and expression analysis was performed by qRT-PCR: (b) TA and DNp73; (c) Cdh1, Snail, Nanog, Epcam, Lin28 and Pecam1. Expression of the indicated genes was normalized to 18S and set to 1 for non-transfected MEFs in each graph (t=0). Analysis was performed with data from two independent experiments, with at least three biological replicates from the indicated genotypes, with two replicates per sample. Mean±S.E.M. are represented, equal variance. Student's t-test was performed to evaluate statistical differences. *P<0.05, **P<0.01, ***P<0.001

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