Figure 3 | Cell Death & Disease

Figure 3

From: p73 is required for appropriate BMP-induced mesenchymal-to-epithelial transition during somatic cell reprogramming

Figure 3

p53 deficiency could not fully counteract p73 requirement during reprogramming. Expression kinetics analysis of MET and pluripotency markers during the p53KO and DKO-MEFs reprogramming process. RNA samples were collected at the indicated days and expression analysis was performed by qRT-PCR: Cdh1, Snail, Nanog, Epcam, Lin28 and Pecam1. Expression of the indicated genes was normalized to 18S and set to 1 for non-transfected MEFs in each graph (t=0). Analysis was performed with data from two independent experiments, with at least three biological replicates from the indicated genotypes (two biological replicates in the case of p53KO cells), with two replicates per sample. Mean±S.E.M. are represented, equal variance. Student's t-test was performed to evaluate statistical differences; *P<0.05, **P<0.01, ***P<0.001

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