Figure 5
From: LRP6 is identified as a potential prognostic marker for oral squamous cell carcinoma via MALDI-IMS
Expression level of LRP6 was associated with the proliferation ability of OSCC cells. (a) Expression of LRP6 in normal oral squamous cell line and several OSCC cell lines was examined by immunoblot. (b) The relative mRNA level of LRP6 in these cell lines was examined by quantitative PCR (Q-PCR). (c and d) HSC-3 and Cal-27 cell lines were transfected with LRP6-specific small interfering RNAs (siRNAs), and the protein level was detected via immunoblot (c) or Q-PCR (d). (e) HSC-3 and Cal-27 cell lines were transfected with LRP6-specific siRNAs and cell proliferation was detected via the colony formation assay. (f) HSC-3 and Cal-27 cell lines were transfected with LRP6-specific siRNAs, and proliferation of HSC-3 and Cal-27 cell lines was detected by the Cell Counting Kit-8 (CCK8) assay
