Figure 5
From: Abcb1a and Abcb1b genes function differentially in blood–testis barrier dynamics in the rat
Changes in the steady-state mRNA levels of Abcb1a and Abcb1b and protein levels of FAKs following treatment of Sertoli cells with F5-peptide and its removal. Sertoli cells cultured at 0.5 × 106 cells/cm2 on Matrigel-coated multiwell plates were treated with F5-peptide as depicted in Figure 3b. Abcb1a and P-glycoprotein showed a drastic reduction in the mRNA or protein level after 24 h-F5-peptide treatment, respectively, followed by a significant recovery at 12 h after F5-peptide removal. The mRNA level of Abcb1b was not affected by F5-peptide but increased after its removal. The protein level of MRP1 and FAK remained unchanged during and after F5-peptide treatment. The p-FAK-Tyr407 and p-FAK-Tyr397 involved FAK signaling pathway was antagonistically inhibited or induced during F5-peptide treatment and after its removal. qPCR results and representative immunoblots are shown in (a) and (b), respectively, where n=3–4. The densitometry of the proteins is analyzed and summarized in (c). *P<0.05; **P<0.01; statistical difference from the Ctrl group was analyzed by ANOVA with a post hoc Tukey/Kramer test
