Figure 2
From: NDRG1 inhibition sensitizes osteosarcoma cells to combretastatin A-4 through targeting autophagy
Combination treatment of CA-4 and CQ enhances antitumor activity in OS cells. (a) SJSA and MG63.2 cells treated with CA-4 alone, CQ alone, or CA-4/CQ in combination. Cell viability was determined by cell viability assay. (b) CA-4/CQ combination induced significantly synergistic effect in SJSA and MG63.2 cells by using CalcuSyn software (Cambridge, UK). (c) SJSA and MG63.2 were co-treated with CA-4 and CQ, and apoptosis was indicated as sub-G1 population detected by flow cytometry. Bar graphs showed the percentages of sub-G1. (d) Cells were treated as in c, and the whole-cell lysates were subjected to immunoblotting of cleaved PARP, cleaved caspases and GAPDH. (e) The protein bands in (d) were quantified and normalized according GAPDH. The data were presented as mean±S.D (*P<0.05, n=3)
