Figure 6
From: NDRG1 inhibition sensitizes osteosarcoma cells to combretastatin A-4 through targeting autophagy
NDRG1 knockdown sensitizes CA-4 and CQ combination treatment-induced apoptosis in OS cells. (a) Cells transfected with control nontarget or NDRG1 siRNA treated with CA-4 alone, CQ alone or CA-4/CQ in combination. Cell viability was determined by cell viability assay. (b) Apoptosis was indicated as sub-G1 population detected by flow cytometry. (c) Cells were harvested, and then the whole-cell lysates were subjected to immunoblotting of cleaved PARP, cleaved caspase 3 and GAPDH. (d) The cleaved PARP and cleaved caspase 3 bands in c were quantified and normalized according GAPDH. The data were presented as mean±S.D (*P<0.05, n=3)
