Figure 4
From: Inhibition of the deubiquitinase USP5 leads to c-Maf protein degradation and myeloma cell apoptosis
K308 and K347 at c-Maf are the target sites of USP5 in preventing c-Maf ubiquitination. (a) HEK293T cells were transfected with a wild-type (WT) plasmid for 48 h, followed by cell lysate preparation and IB with individual antibodies. (b) A Myc-USP5 plasmid was co-transfected into HEK293T cells with specific HA-c-Maf mutant plasmids for 48 h, followed by immunoblotting. (c, d) HEK293T cells were transfected with HA-c-Maf-K308 (c) or HA-c-Maf-K347 (d) along with increased USP5. Forty-eight hours later, cells were harvested for IB to determine the c-Maf protein levels. (e) HEK293T cells were transfected with HA-tagged wild-type (WT) or mutant c-Maf, followed by the IP/IB assays with indicated antibodies
