Figure 7
From: Inhibition of the deubiquitinase USP5 leads to c-Maf protein degradation and myeloma cell apoptosis
USP5 modulates c-Maf transcriptional activity. (a) The MARE.Luci or mtMARE.Luci plasmids were co-transfected with c-Maf or USP5 for 48 h followed by luciferase activity measurements and IB assays. (b) Cells transfected with c-Maf and USP5 were treated with WP1130 for 12 h, followed by luciferase activity measurement and IB assays. (c) RPMI-8226 and LP1 cells were treated with WP1130 for 12 h, followed by total RNA extraction and RT-PCR analysis. (d) LP1, RPMI-8226, and U266 cells were transfected with siUSP5 for 48 h, followed by RT-PCR for indicated genes
