Figure 9
From: Inhibition of the deubiquitinase USP5 leads to c-Maf protein degradation and myeloma cell apoptosis
Suppression of the USP5/c-Maf axis leads to MM cell apoptosis. (a, b) MM cell lines RPMI-8226, LP1 and U266 were treated with WP1130 at indicated concentrations for 12 h, followed by IB assay (a) or Annexin V/PI staining and FACS assay (b). (c, d) RPMI-8226 (c) and LP1 (d) cells were treated with WP1130 for 12 h, followed by IP/IB assay. (e–f) RPMI-8226 cells were transfected with siUSP5 or scrambled siRNA for 72 h, followed by WP1130 treatment for 12 h. The cell lysates were subjected to IB assay (e). The ratios of c-Maf over GAPDH from (e) were reported. N.S., not significant
