Table 1 Primers for constructs of USP5 domains
From: Inhibition of the deubiquitinase USP5 leads to c-Maf protein degradation and myeloma cell apoptosis
Domain | a.a. region | Size (bp) | Primers (5'-3') |
|---|---|---|---|
D1 Cryptic ZnF | 1–168 | 504 | F: ATTGGATCCATGGCGGAGCTGAGTGAGGAGGCGCTGCTR: CCGCTCGAGTTATGCCTGCACCTCCTGCTTGCG |
D2 ZnF | 169–289 | 363 | F: ATTGGATCCTGGGATGGGGAAGTACGGCAGR: CCGCTCGAGTTATGTCTTCTGCATCTTCAGCATGTC |
D3 C-Box | 290–624 | 1005 | F: ATTGGATCCGACAAGACGATGACTGAGTTGR: CCGCTCGAGTTACGGAGTGACCAGGGGT |
D4 UBA1–UBA2 | 625–749 | 375 | F: ATTGGATCCGATGAGCCCAAAGCGCCCATR: CCGCTCGAGTTAGGAGCCCGGCCCACTAGA |
D5 H Box | 750–835 | 258 | F: ATTGGATCCACAAGCGCAGCAGCCGACR: CCGCTCGAGTTAGCTGGCCACTCTCTGGTAGAAGTAGAT |
