Figure 2 | Cell Death & Disease

Figure 2

From: Trehalose ameliorates oxidative stress-mediated mitochondrial dysfunction and ER stress via selective autophagy stimulation and autophagic flux restoration in osteoarthritis development

Figure 2

Trehalose induces autophagy in chondrocytes. (a–f) The chondrocytes were treated with 0, 25, 50, 100 or 200 mM trehalose for 24 h or 100 mM trehalose for 0, 6, 12, 24 or 48 h, and protein levels of Atg7, Atg12-5, Atg3, LC3, Beclin-1 and p62 were detected. (g and h) The chondrocytes were treated with TBHP, trehalose or CQ, and the levels of LC3, p62 and ubiquitin were detected. (i) p62 accumulation was examined by immunofluorscence staining (bar: 10 μm). All data represent mean±S.D (n=5). **P<0.01, *P<0.05

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