Figure 2
From: Hispolon suppresses metastasis via autophagic degradation of cathepsin S in cervical cancer cells
Effects of hispolon on proteinases expression which influenced the ability of metastasis in cervical cancer cells. (a) Collected the cell protein lysate and detected the protein expression by Human Proteinases Array. (b) The cells were seeded on 6 cm plates and treated with hispolon (0, 25, 50, 100 μM) for 24 h. Then the cell protein lysate was collected and analyzed by western blot assay. (c,d) After treatment with hispolon (0,100 μM) in 0, 2, 4, 6, 8, 12 and 24 h. The cell lysates were collected in cell lysis buffer and analyzed by Cathepsin S activity assay. (e,f) The cells were treated with hispolon (100 μM) or Cathepsin S inhibitor Z-FL-COCHO (20 μM) for 24 h, then analyzed by western blot and migration assay. (g,h) HeLa and SiHa cells transfected with the siRNA of Cathepsin S for 48 h and analyzed by western blot and migration assay. (i,j) HeLa cells transfected with pCMV3-CTSS-His plasmid 5 μg for 6 h and treated with hispolon for 24 h, then analyzed by Western blot and migration assay. (The values represented the mean±S.D. from three determinations per condition repeated three times. *P<0.05 compared with untreated. #P<0.05 compared with hispolon (100 μM))
