Figure 3
From: Hispolon suppresses metastasis via autophagic degradation of cathepsin S in cervical cancer cells
Effects of hispolon-induced autophagy in cervical cancer cells. (a) Transformation of LC3-I to LC3-II after HeLa and SiHa cells were treated by various hispolon concentrations (0, 25, 50 and 100) for 24 h and the results analyzed by western blot. (b,c) To detect the AVOs by acridine orange stained after hispolon treatment and analyzed by microscopy or flow cytometry. (d) GFP-LC3 dots were observed after the cells transfected with the plasmid and hispolon treatment. (e,f) To determine that normal flux autophagy was induced by hispolon in cervical cancer cells. These results were analyzed by western blot
