Figure 6
From: Hispolon suppresses metastasis via autophagic degradation of cathepsin S in cervical cancer cells
Hispolon-mediated autophagy and suppressed cell metastasis via P-ERK pathway in cervical cancer cells. (a) HeLa and SiHa cells were treated with various hispolon concentrations and analyzed MAPK pathway by western blot. (b) The cells were pre-treated with U0126 and co-treated with hispolon then the results were observed by wound healing assay. (c,d) HeLa and SiHa cells were treated as the same as (b) and the results were determined by migration and invasion assay. (e,f) The cells were transfected with ERK-siRNA for 48 h and treated with hispolon for 24 h. The results were detected by migration assay and western blot. (g) HeLa cells were transfected with ERK-siRNA for 48 h and pre-treated with chloroquine 1 h and co-treated with hispolon for 23 h then analyzed by western blot assay. (h) The cells were pre-treated with U0126 for 1 h and then co-treated with hispolon for 23 h. These results were detected by western blot. (i) HeLa and SiHa cells were treated with the MEK inhibitor U0126 for 1 h and co-treated with hispolon. To detect the AVO and observed by fluorescent microscopy. (The values represented the mean±S.D. from three determinations per condition repeated three times. *P<0.05)
