Figure 4

YWHAZ is associated with miR-451 expression. (a) mRNA expression of YWHAZ in tumor tissues and adjacent normal tissues of BC patients was revealed by mRNA-seq provided by TCGA. (b) Scatterplot depicts a significant inverse correlation between miR-451 and YWHAZ mRNA expression. (c and d) Real-time RT-PCR assay and (e and f) western blotting analysis were performed to detect the expression of YWHAZ at the mRNA and protein levels, in PR cells treated with miR-451 mimics and parent cells treated with miR-451 inhibitor,respectively. (g) Sequences of wild-type and. mutant target sites for miR-451 in YWHAZ are shown. (h) Luciferase reporter assays were performed to identify the binding of miR-451 to YWHAZ 3′-UTR in PR cells and their parent cells. MT, mutation; WT, wild type. Bars represent mean±S.D. and asterisks denote a significant difference (*P<0.05; **P<0.01 and ***P<0.001). Student’s t-test. Data is representative of at least three independent experiments