Figure 1
HCMV infection induces oncogenic properties in non-transformed HFFs. (a) GFP expression was observed under the fluorescence microscope at 24 h post infection (hpi) with GFP-tagged WT-HCMV (H-WT) in HFFs. (b) Culture media color change (red to yellow) was visually observed on day 6 post infection (dpi), in HFFs infected with H-WT. (c) Cell viability (metabolic activity) of mock or H-WT-infected HFFs was quantified by MTT assay, on 6 dpi. (d) Transcript and (e) protein of MKi67 was quantified by qPCR and flow cytometer respectively, in mock, H-WT-infected HFFs, (f, left, right, bottom) flowcytometry was done for mock and H-WT-infected HFFs by staining them with propidium iodide (PI, shown in x axis) on 3 dpi, to detect cell cycle stages upon HCMV infection and (g) Anti-apoptotic gene (bcl2, birc3 and prkce) status was compared by qPCR in mock or H-WT infected HFFs on 6 dpi. Shown results are the representative of three (a–d, g) or two (e, f) independent experiments. (f) Statistical analysis was done with data of two independent experiments. Differences were considered statistically significant with a *P-value<0.05, **P-value<0.01 and ***P-value<0.001, ns, non-significant difference (P-value>0.05)
