Figure 5 | Cell Death & Disease

Figure 5

From: Essential role of HCMV deubiquitinase in promoting oncogenesis by targeting anti-viral innate immune signaling pathways

Figure 5

Nuclear Localization Sequence of UL48N is dispensable for UL48-DUB activity (a) Schematic representation of UL48N and its NLS-mutant (UL48NΔNLS). (b) The cellular localization of mCherry-tagged UL48N and UL48NΔNLS was observed under the fluorescence microscope at × 63. Nucleus was stained with Hoechst 33342. (c) DUB activity of UL48N and UL48NΔNLS was compared, by observing the ubiquitination status of whole cell lysates (WCL), upon overexpressing UL48N and UL48NΔNLS in HEK293 cells, as indicated. (d, e) Luciferase assay, was done for IFNα4 or IFNβ promoter by co-transfection of MyD88 or STING and UL48N or UL48NΔNLS expression plasmids as depicted. (f, left, right) UL48N or UL48NΔNLS was over-expressed in IMR32 cells, wound was created and wound-healing was observed at 48 h and width of the wound was measured. Shown results are the representative of Single (b, c) or two (d, e and f) independent experiments. Differences were considered statistically significant with a ***P-value<0.001

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