Figure 5 | Cell Death & Disease

Figure 5

From: Activation of miR-34a impairs autophagic flux and promotes cochlear cell death via repressing ATG9A: implications for age-related hearing loss

Figure 5

ATG9A is targeted by miR-34a in the regulation of autophagy. (a and b) HEI-OC1 cells transfected with miR-34a mimics and its control were subjected to western blot analysis for ATG9A. (c and d) Western blot and densitometry analysis performed after the transfection of miR-34a inhibitor and inhibitor control. (e–h) HEI-OC1 cells were transfected with si-control and si-ATG9A. Seventy-two hours post-transfection, total protein was harvested and subjected to western blot analysis for ATG9A, LC3-II and p62, and β-actin was used as a loading control. Quantification of band intensities normalized to β-actin and relative to control are shown below respective blots. Fluorescence images of mRFP-GFP-LC3 in HEI-OC1 cells treated with a si-control (i) and si-ATG9A (j) with a nutrition-free medium for 6 h before fixation. Quantity analysis of yellow and red puncta was detected (k). Scale bars: 10 μm. Date were represented as the mean±S.E.M. obtained from four independent experiments. *P<0.05

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