Figure 1
RTN1-C is upregulated during cerebral ischemia/reperfusion. Brain samples were collected from normal and ischemia/reperfusion (I/R) rat brain tissues. The male SD rats (n=3) were subjected to a middle cerebral artery occlusion (MCAO) for 2 h, followed by 12 or 24 h of reperfusion. (a) Representative western blot of three splice variants of RTN1 in the brain tissue samples from sham-operated and I/R rats. (b) Immunofluorescent staining of RTN1-C on brain sections from I/R rats. The upper panel is brain sections from sham-operated groups; the middle panel is the brain section from the group with MCAO for 2 h followed by 12 h of reperfusion; the lower panel is the brain section from the group with MCAO for 2 h followed by 24 h of reperfusion. Immunofluorescence was performed with anti-RTN1-C (green). DAPI was used to stain the nuclei (blue). The arrows highlight the RTN1-C expression. Scale bar=20 μm. (c) Representative immunofluorescent staining of RTN1-A on the brain sections of I/R rats. Immunofluorescence was performed with anti-RTN1-C (green). DAPI was used to stain the nuclei (blue). Scale bar=20 μm. (d) Representative western blot of RTN1-C in the brain tissue samples from I/R, ischemic postconditioning (IPC), Remifentanil postconditioning (RPC), and sham-operated rats
