Figure 3
RTN1-C induces ER stress-associated apoptosis during OGD/R. (a) Representative western blot analysis of Grp78, cleaved-caspase-12, CHOP and cleaved-caspase-3 in N2a cells exposed to OGD/R treatment or normal conditions, after transfection with RTN1-C-FLAG or shRTN1-C. GAPDH was used as a loading control. (b) Histogram showing quantification of images in a. The results were normalized to GAPDH expression, and the quantitative data were expressed as the mean±S.D. from different assays (n=3). *P<0.05; **P<0.01; ***P<0.001. (c) Representative western blot analysis of Grp78, cleaved-caspase-12, CHOP and cleaved-caspase-3 in N2a cells transfected with RTN1-C-GFP or GFP vector in the absence or presence of 4-PBA for 6 h before OGD/R treatment. GAPDH was used as a loading control. (d) Histogram showing quantification of images in c. The results were normalized to GAPDH expression, and the quantitative data were expressed as the mean±S.D. from different assays (n=3). *P<0.05; **P<0.01; ***P<0.001
