Figure 5

HSF4 stabilizes and orients p53 in the nucleus to initiate. (a) p53 is retained in the nucleus by HSF4. HLECs were transfected with GFP-tagged HSF4 (green) plasmids and negative control GFP vectors, respectively. Cells were harvested 48 h after transduction followed by immunofluorescence stains with anti-p53 antibody. Arrows indicated the overlap of the p53 signals (red) and the HSF4 signals (green). Scale bar: 10 μm. (b) The nuclear distribution of p53 was increased when HSF4 was overexpressed in HLECs. (c) Nuclear-oriented p53 was decreased when HSF4 was silenced in HLE cells. The cytoplasmic and nuclear proteins were separated and subjected to western blot detection. Tubulin and FBL were used as specific markers for the cytoplasm and nuclear components, respectively