Figure 7

hsf4 loss can be partially rescued by overexpression of hsf4, p53 and fas. (a) Three-M-old WT and hsf4^null zebrafish lenses were isolated and subjected to protein extraction. Western blot results indicated that p53 and the activation of caspase3 was significantly decreased in the hsf4null zebrafish. (b) Western blot analysis of the proteins from 6-M-old WT and hsf4^null zebrafish lenses. The results revealed that p53 and cleaved-caspase3 were also obviously decreased in the 6-M-old hsf4^null zebrafish lens. (c) Overexpression of hsf4, p53 and fas could partially rescue the denucleation defect in hsf4null zebrafish lens. The hsf4, p53 and fas mRNA was microinjected into the hsf4^null zebrafish embryos. The denucleation status was checked at 3 and 5d, respectively, through the DAPI staining. Obviously, decreased numbers of nuclei were presented in the lens injected with hsf4, p53 and fas mRNA at both 3 and 5d. Scale bar, 20 μm