Figure 4 | Cell Death & Disease

Figure 4

From: miRNA-223 upregulated by MYOD inhibits myoblast proliferation by repressing IGF2 and facilitates myoblast differentiation by inhibiting ZEB1

Figure 4

miR-223 promotes myoblast differentiation. (a) Relative miR-223 expression during CPM differentiation. (b) Relative miR-223 expression during qm-7 differentiation. (c) Relative expression of muscle differentiation marker genes after CPM transfected with miR-223 and NC. (d) Relative expression of muscle differentiation marker genes after qm-7 transfected with miR-223 and NC. (e) Relative expression of muscle differentiation marker genes after CPM transfected with miR-223 inhibitor and NC inhibitor. (f) Relative expression of muscle differentiation marker genes after qm-7 transfected with miR-223 inhibitor and NC inhibitor. (g) MyHC staining of CPM at 72 h after transfection of miR-223 or NC mimic. (h) Myotube area (%) at 72 h after transfection of miR-223 or NC mimic. Results are shown as the mean±S.E.M. of three independent experiments. Independent sample t-test was used to analysis the statistical differences between groups. *P<0.05; **P<0.01

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