Figure 2
From: Inactivation of Lsd1 triggers senescence in trophoblast stem cells by induction of Sirt4
Lsd1-controlled transcription regulates metabolic pathways and aging. (a, d and e) Comparison of TSCs cultured for 24 h in the presence of solvent (Ctrl) or Lsd1 inhibitor 1 (Lsd1i-1) by RNA-sequencing (RNA-seq). (a) Significantly differentially transcribed genes with more than 50 reads are depicted as red dots. RNA-seq was done with two samples. (b) Pie chart displaying the genomic distribution of Lsd1 peaks in TSCs determined by ChIP-seq. ChIP-seq was performed with a single sample. (c) Read coverage for genomic Lsd1-binding sites in TSCs between transcription start (TSS) and transcription end site (TES) including 2 kb at the 5’ and 3’ site. (d) Direct Lsd1 targets were determined by intersection of Lsd1 promoter-occupied and differentially expressed genes. (e) Enrichment analyses for promoter-occupied and differentially expressed Lsd1 targets according to P-value. Statistical analysis was performed using MACS (ChIP-seq) and edgeR (RNA-seq)
