Figure 5
Downregulation of UHRF1 promotes RIP3 expression depending on Sp1. (a) RKO stably expressing UHRF1-shRNA and Sp1-shRNA cells were generated as described in the Materials and Methods, and western blotting analysis of lysates from the indicated cell lines showing RIP3, UHRF1, Sp1 and β-actin levels. (b) The indicated cells lines were treated as indicated for 48 h. Cell viability was determined by measuring ATP levels. *P<0.05. (c) HCT116-RIP3 stably expressing Sp1-shRNA cells were generated as described in the Materials and Methodds. The indicated cell lines were treated as indicated for 48 h. Cell viability was determined by measuring ATP levels. (d) Western blotting analysis of lysates from the indicated cells were showing Sp1, RIP1, RIP3, MLKL and β-actin levels
