Figure 2 | Cell Death & Disease

Figure 2

From: Long non-coding RNA SNHG20 promotes non-small cell lung cancer cell proliferation and migration by epigenetically silencing of P21 expression

Figure 2

Effects of SNHG20 on NSCLC cell proliferation in vitro. (a) qRT-PCR analysis of SNHG20 expression in normal human bronchial epithelial cells (16HBE) and NSCLC cells. (b) qRT-PCR analysis of SNHG20 expression in control (scrambled), si-SNHG20 1#, si-SNHG20 2# and si-SNHG20 3# treated NSCLC cells. (c) MTT assays were used to determine the viability of si-SNHG20-transfected SPC-A1 and A549 cells. (d) Colony formation assays were performed to determine the proliferation of si-SNHG20-transfected SPC-A1 and A549 cells. Colonies were counted and captured. (e) EdU staining assays were performed to determine the growth of si-SNHG20 transfected SPC-A1 and A549 cells. Representative images and data are based on three independent experiments. Data are presented as the mean±S.E.M. *P<0.05, **P<0.01

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