Figure 3

Effect of SNHG20 on NSCLC cell apoptosis, cell cycle and migration in vitro. (a and b) SPC-A1 and A549 cells were stained and analyzed by flow cytometry. LR, early apoptotic cells; UR, terminal apoptotic cells. (c and d) Flow cytometry showing significant decreases or increases in the proportion of cells in the S or G1 phase, respectively, when SNHG20 was silenced in SPC-A1 and A549 cells. (e) Transwell assays were performed to investigate changes in migratory abilities of SPC-A1 and A549 cells. Data are presented as the mean±S.E.M. All experiments were performed in triplicate with three technical replicates. *P<0.05, **P<0.01