Figure 6 | Cell Death & Disease

Figure 6

From: Long non-coding RNA SNHG20 promotes non-small cell lung cancer cell proliferation and migration by epigenetically silencing of P21 expression

Figure 6

Downregulation of P21 promotes A549 cell proliferation and SNHG20 negatively regulates expression of P21. (a) A549 cells were transfected with si-P21. (b and c) MTT and colony formation assays were used to determine the cell viability of si-P21-transfected A549 cells. Experiments were performed in triplicate. (d) Flow cytometry assays were performed to analyze cell-cycle progression of A549 cells transfected with si-P21. The bar chart represents the percentage of cells in G0–G1, S or G2–M phases, as indicated. (e and f) MTT and colony formation assays were used to determine the cell viability of si-SNHG20 and si-P21 co-transfected A549 cells. (g) P21 expression was analyzed by western blotting. Values are shown as the mean (S.D.) from three independent experiments. *P<0.05, **P<0.01

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