Figure 3

FGF10 reduces apoptosis via activation of the PI3K/Akt pathway. After SCI in rats, (a and b) TUNEL assay was performed in each group at 7 days after SCI (scale bar: 50 μm). (c and d) Western blots and quantification data of cleaved-caspase 3, Bax and Bcl-2 of each group at 3 days after surgery. (e) Double immunofluorescence of NeuN (red) and cleaved-caspase 3 (green) of each group at 3 days after surgery (scale bar: 50 μm). Data represent the mean±S.D. Significant differences between the treatment and SCI groups are indicated as *P<0.05, **P<0.01, ***P<0.001, n=5